Microbial Bacterial Production in Lakes at North Temperate Lakes LTER 2000 - 2002

Net production of bacteria passing a 70 micron mesh, or bacteria passing a 1 micron
mesh, calculated incorporation of 3H labeled leucine into cell proteins. Method based on
microcentrifuge method of Smith, David C., and Farooq Azam. 1992. A simple, economical
method for measuring bacterial protein synthesis rates in seawater using 3H-leucine, Marine
Microbial Food Webs 6(2):107-114. Nanomolar treatments refer to leucine concentration for
calculation of isotopic dilution and necessary leucine concentration to saturate uptake into
bacterial cells. For a good example on how to calculate isotopic dilution see Pace. Michael
L., and Jonathan J. Cole. Primary and bacterial production in lakes: are they coupled over
depth? Plankton Research 16(6):661-672. Sampling Frequency: fortnightly during ice-free
season - every 6 weeks during ice-covered season Number of sites: 4

本数据集测定通过70微米筛网（70 micron mesh）的细菌，或通过1微米筛网（1 micron mesh）的细菌的净生产量，该指标通过将3H标记亮氨酸（3H-labeled leucine）掺入细菌细胞蛋白质的方式计算得到。该方法基于Smith、David C.与Farooq Azam于1992年提出的微量离心法，相关研究论文为《使用3H-亮氨酸测定海水中细菌蛋白质合成速率的简便经济型方法》，刊载于《海洋微生物食物网（Marine Microbial Food Webs）》6(2):107-114。纳摩尔浓度处理指的是用于计算同位素稀释（isotopic dilution）的亮氨酸浓度，以及使细菌细胞对亮氨酸的摄取达到饱和所需的亮氨酸浓度。关于同位素稀释计算的优质示例可参考Pace、Michael L.与Jonathan J. Cole的研究：《湖泊中的初级生产与细菌生产：二者在垂直深度上是否存在耦合？》，刊载于《浮游生物研究（Plankton Research）》16(6):661-672。采样频率：无冰期每两周采样一次，冰封期每6周采样一次。采样位点数量：4个。