Direct reprogramming of adult adipose-derived regenerative cells toward cardiomyocytes using six transcriptional factors I

It is widely accepted that adipose-derived regenerative cells (ADRCs) can differentiate into mesodermal lineage cells. However, reprogramming adult ADRCs into mature cardiomyocytes is challenging. We investigated the induction of myocardial differentiation in ADRCs via direct reprogramming using lentiviral gene transfer. We compared gene expression profiling from RNA sequencing of culture ADRCs and embryonic heart organ, and examined candidate transcriptional factors. Overall design: Comparative gene expression profiling analysis of RNA-seq data for culture ADRCs from adult mouse adipose tissue, and heart tissue from embryonic (E11.5) mouse.