Reducing the number of accepted species in Aspergillus series Nigri

The Aspergillus series Nigri contains biotechnologically and medically important species. They can produce hazardous mycotoxins, which is relevant due to the frequent occurrence of these species on foodstuffs and in the indoor environment. The taxonomy of the series has undergone numerous rearrangements, and currently, there are 14 species accepted in the series, most of which are considered cryptic. Species-level identifications are, however, problematic or impossible for many isolates even when using DNA sequencing or MALDI-TOF mass spectrometry, indicating a possible problem in the definition of species limits or the presence of undescribed species diversity. To re-examine the species boundaries, we collected DNA sequences from three phylogenetic markers (benA, CaM and RPB2) for 276 strains from series Nigri and generated 18 new whole-genome sequences. With the three-gene dataset, we employed phylogenetic methods based on the multispecies coalescence model, including four single-locu..., ,

曲霉属黑曲霉系（Aspergillus series Nigri）包含诸多兼具重要生物技术与医学价值的物种。该类群可产生有害真菌毒素（mycotoxins），鉴于其频繁在食品及室内环境中出现，该特性具有重要研究与应用价值。该系的分类学历经多次修订调整，目前公认该系包含14个物种，其中多数被视为隐存物种。然而，即便采用DNA测序或基质辅助激光解吸电离飞行时间质谱（MALDI-TOF mass spectrometry）技术，多数分离株仍难以完成物种水平的鉴定，甚至无法实现鉴定，这暗示物种界限的定义可能存在疏漏，或是存在尚未被描述的物种多样性。为重新审视该类群的物种界限，我们从黑曲霉系的276株菌株中获取了3个系统发育标记基因（benA、CaM及RPB2）的DNA序列，并新生成了18株菌株的全基因组序列。基于该三基因数据集，我们采用了基于多物种溯祖模型的系统发育分析方法，涵盖4个单基因……