TMI-1 inhibits cell viability and induces caspase-3/7 activity in different tumoral mammary cell lines.

Breast tumoral and non tumoral epithelial cells as well as primary endothelial and fibroblastic cells were treated with TMI-1 (0.3125–20 µM) for 5 days. Cell viability assay was done with the Alamar blue staining kit. Caspase-3/7 activation was measured as in Figure 2. (+): Caspase-3/7 activity level increases (from 2 to 10 fold from control). (−): Caspase-3/7 activity level is invariant. Experiments were done in triplicate for each concentration tested. Molecular subtype status of each cell line and ED50 values are indicated. These results are representative of at least three independent experiments.