Centromeric R loops contribute to defects in kinetochore assembly and chromosomal instability

We mapped ssDNA formation in WT and hpr1delta cells at permissive (30C) and non-permissive (37C) temperature. We showed that hpr1 cells accumulate ssDNA show elevated levels of ssDNA at centromeric regions. Additionally, we show replication fork-associated ssDNA at origins of replication is more confined to the origins in hpr1 cells than WT cells, suggesting a defect in replication fork progression.我们在许可温度（30℃）与非许可温度（37℃）条件下，对野生型（Wild Type, WT）及hpr1delta细胞中的单链DNA（single-stranded DNA, ssDNA）形成情况进行了图谱绘制。研究发现，hpr1delta细胞会发生单链DNA累积，且在着丝粒区域的单链DNA水平显著升高。此外，相较于野生型细胞，hpr1delta细胞中与复制叉相关的复制起始位点单链DNA更局限于起始位点区域，这表明其复制叉行进过程存在缺陷。