Nanopore RNA sequencing in S. cerevisiae strains defective for decapping and deadenylation

RNA turnover in eukaryotes is triggered by decapping, believed to be activated mostly after deadenylation. To investigate simultaneous changes in RNA levels and poly(A) tail size we performed Nanopore RNA sequencing on total RNA extracted from S. cerevisiae strains in which the decapping enzyme Dcp2, or the deadenylation factors Ccr4 and Pop2 were depleted through an inducible degron system. Two fully independent replicate experiments were performed for wild type and the two mutant conditions. Nanopore RNA-seq data from Dcp2_degron and CCR4-POP2_degron induced strains compared to the corresponding wild type BY4741 reference strain.