smFRET and biochemical data for the study of PPR binding to single-stranded RNA

Dataset contains (predominantly) pre-processed data for single-molecule FRET (smFRET) and other biochemical data (e.g., SDS-PAGE gels, surface plasmon resonance experiments, FPLC chromatograms). For smFRET experiments, the conformation of individual pentatricopeptide repeat proteins (PPRs) was monitored in real-time in the presence of various single-stranded RNA target sequences (with data provided as .dat files). Also contained in this repository are data for three-colour FRET experiments, combining a FRET probe on the PPR protein and a fluorophore labelled RNA oligonucleotide (data provided as .ascii files). Data is published as a pre-print in BioRxiv (https://www.biorxiv.org/content/10.1101/2024.04.22.590477v1.abstract). 

Note: data reported in some supplementary figures are provided in Figure 2, 3 and 4 datasets.