The role of EHMT2 in Uveal melanoma progression [EHMT2_Inhibitors]

EHMT2/G9a expression is elevated in many cancer types including uveal melanoma. Here in the present study, we have identified pharmacological inhibition of EHMT2/G9a in MP41 cells and Mel92.1 cells promote the sensitivity to NK cells in vitro. By keeping this in mind, we have performed RNA-seq of inhibition of EHMT2/G9a validated cells to identify the differentially regulated genes from various pathways that are responsible for promoting resistance to NK cell mediated cytotoxicity. Overall design: MP-41 and Mel 92.1 cells were treated with EHMT2/G9a inhibitor UNC0642 & A366, samples were harvested and were used to prepare the total RNA, followed by RiboRNA depletion with random priming and RNASeq Illumina NextSeq 500 platform, with three biological replicates for each sample.