Expression profiling of E14 cells overexpressing Hk2/Pkm2
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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Purpose: In this study, we compared transcriptome profiling (RNA-seq) between normal mouse embryonic stem cell (E14) and Hexokinase2 (Hk2)/ Pyruvate Kinase M2 (Pkm2) overexpressed E14 cell.Result: Using an optimized data analysis workflow, we mapped over 4 billion sequence reads per sample to the mouse genome (build mm9) and identified 28698 transcripts in 5 samples.Conclusion: Our study represents the first detailed analysis of Hk2/ Pkm2 overexpressed E14 cell transcriptomes, generated by RNA-seq technology
研究目的:本研究针对正常小鼠胚胎干细胞(E14,mouse embryonic stem cell)与己糖激酶2(Hexokinase2, HK2)/丙酮酸激酶M2(Pyruvate Kinase M2, PKM2)过表达的E14细胞,开展RNA-seq转录组谱对比分析。
结果:本研究采用优化后的数据分析流程,将每个样本中逾40亿条序列读段比对至小鼠基因组(组装版本mm9),并在5个样本中鉴定出28698条转录本。
结论:本研究为首例基于RNA-seq技术的己糖激酶2/丙酮酸激酶M2过表达E14细胞转录组的精细分析。
提供机构:
Seoul National University
创建时间:
2022-02-20



