Cape Darnley Early-Autumn Phytoplankton Bloom, March 2012

These data relate to a large-scale early-autumn phytoplankton bloom that occurred off Cape Darnley, East Antarctica, in March 2012. The bloom was detected by Dr Jan Lieser (Antarctic Climate and Ecosystems Cooperative Research Centre, ACE-CRC) through MODIS satellite and was opportunistically sampled from RSV Aurora Australis using the uncontaminated seawater line. Samples were analysed for protist species and abundances using light and scanning electron microscopy, and pigment analyses were conducted using high performance liquid chromatography. Additional water samples were taken for dissolved nutrient analyses. Specific details of the files are:Cape Darnley Protist CountsSamples were preserved with 1 % vol:vol Lugols iodine and stored in glass bottles in the dark at 4 degrees C. Protists were identified and counted using phase and Nomarski interference optics using Olympus IX71 and IX81 inverted microscopes at 400X to 640X magnification. Bright field optics were also used to discriminate taxa that contained chloroplasts. Protistan taxa were counted in 20 randomly chosen fields of view, except for highly abundant taxa that were counted in a subset of the field of view defined by an ocular quadrant (Whipple grid). Cell biovolumes and carbon conversion statistics were used to calculate the cell biomass of protistan taxa/groups.Cape Darnley Fluorometer CalibrationFluorometer measurements from the ships underway system were calibrated using chlorophyll a readings determined through high performance liquid chromatography. A linear relationship was established between fluorometer v HPLC chlorophyll a measurements at the same sites. The linear equation was then used to convert all underway fluorometry data from the voyage.Cape Darnley Bloom HPLC Pigments CHEMTAX summaryMajor phytoplankton groups at each site determined through analysis of pigments using high performance liquid chromatography and CHEMTAX. Methods were according to that of Wright et al. (2010).Cape Darnley Bloom NutrientsDissolved nutrient concentrations. Samples were analysed by the Department of Primary Industries, Parks, Water and Environment, 18 St. Johns Avenue, Newtown, Tasmania 7008.Cape Darnley Underway Data VOYAGE_04_0_201112Raw underway data from Aurora Australis in the bloom regionCape Darnley Underway Data MapsMaps of the underway data in the bloom region

本数据集关联2012年3月在南极东部达恩利角（Cape Darnley）近海发生的一次大规模初秋浮游植物水华事件。该水华由扬·利瑟博士（南极气候与生态系统合作研究中心，ACE-CRC）借助MODIS卫星发现，并由澳大利亚极光号科考船（RSV Aurora Australis）通过无污染海水采样管线进行机会性采样。研究人员通过光学显微镜与扫描电子显微镜对样本中的原生生物物种组成与丰度进行分析，并采用高效液相色谱（high performance liquid chromatography, HPLC）开展色素分析。此外还采集了额外水样以开展溶解态营养盐分析。

各文件具体说明如下：
达恩利角原生生物计数数据（Cape Darnley Protist Counts）
样本采用体积分数1%的鲁戈氏碘液（Lugols iodine）固定，置于棕色玻璃瓶中，于4℃暗处保存。使用奥林巴斯IX71与IX81型倒置显微镜，以400倍至640倍放大倍率，采用相差光学与Nomarski干涉光学（Nomarski interference optics）对原生生物进行鉴定与计数；同时采用明场光学方法区分含有叶绿体的类群。原生生物类群计数在20个随机选取的视野中完成；对于丰度极高的类群，则采用目镜象限（Whipple grid，即惠普勒网格）限定的部分视野进行计数。研究人员通过细胞生物体积与碳转化系数计算原生生物类群/类组的细胞生物量。

达恩利角荧光计校准数据（Cape Darnley Fluorometer Calibration）
采用高效液相色谱测定的叶绿素a浓度，对科考船走航系统的荧光计测量数据进行校准。在相同采样站位处，建立了荧光计测量值与高效液相色谱叶绿素a测量值之间的线性关系。随后利用该线性方程对本次航次所有走航荧光测量数据进行转换。

达恩利角水华高效液相色谱色素及CHEMTAX汇总数据（Cape Darnley Bloom HPLC Pigments CHEMTAX summary）
通过高效液相色谱色素分析结合CHEMTAX软件，确定各站位的主要浮游植物类群；分析方法参照Wright等人（2010年）的研究方案。

达恩利角水华营养盐数据（Cape Darnley Bloom Nutrients）
溶解态营养盐浓度数据。样本由塔斯马尼亚州纽敦市圣约翰大道18号的初级产业、公园、水利与环境部（Department of Primary Industries, Parks, Water and Environment）完成分析。

达恩利角走航数据 VOYAGE_04_0_201112
极光号科考船在水华区域采集的原始走航数据。

达恩利角走航数据地图（Cape Darnley Underway Data Maps）
水华区域走航数据相关地图。