Assessment of oxidative stress in saliva of children with dental erosion

ABSTRACT Objective To evaluate oxidative stress in saliva of children with dental erosion as compared to children with no erosion. Methods One single examiner, trained and prepared to make diagnosis of dental erosion according to the Basic Erosive Wear Examination index, selected 40 children aged 4 to 6 years, who attended a pediatric dentistry prevention clinic. Two groups were formed - one comprising children with dental erosion (n=22), and another with no dental erosion (n=18). The quantity of dental biofilm was verified using the Simplified Index of Oral Hygiene, and unstimulated saliva was collected for biochemical analyses. The following were assessed in saliva: flow rate, buffering capacity, pH, and total protein concentration. Malondialdehyde levels were also verified to determine oxidative stress and total antioxidant status. Results The quantity of biofilm was smaller in children with mean dental erosion±standard deviation (0.76±0.25), as compared to those with no dental erosion (1.18±0.28). There was no statistical difference in saliva parameters of oxidative stress in children with dental erosion. Conclusion The activity of oxidative stress in saliva did not influence dental erosion process when in its early stages.

摘要
目的：对比牙酸蚀症（dental erosion）患儿与无牙酸蚀症儿童的唾液氧化应激水平。
方法：由1名经培训合格、可依据基本酸蚀磨损检查指数（Basic Erosive Wear Examination index）开展牙酸蚀症诊断的检查者，选取40名4~6岁且就诊于儿童牙科预防门诊的儿童作为研究对象。将受试者分为两组：牙酸蚀症组（n=22）与无牙酸蚀症组（n=18）。采用简化口腔卫生指数（Simplified Index of Oral Hygiene）评估牙菌斑量，采集非刺激性唾液用于生化分析。对唾液的流速、缓冲能力、pH值及总蛋白浓度进行检测，并通过检测丙二醛（Malondialdehyde）水平以评估氧化应激状态与总抗氧化能力。
结果：牙酸蚀症组儿童的牙菌斑量（平均值±标准差：0.76±0.25）低于无牙酸蚀症组（1.18±0.28）。两组儿童唾液的氧化应激相关参数未发现统计学差异。
结论：在牙酸蚀症早期阶段，唾液氧化应激活性并未对其发病进程产生影响。