Identified slow growth mutants.

Summary of growth phenotypes of SG1 –SG12 slow growth mutants, and analysis of the sequences present in each corresponding library plasmid. Growth values represent cell numbers normalized to vector control transfectants on day 4 of growth assays. The corresponding raw data growth assay data are found in Figs 5A, S12A, S12C and S12E). Mutants exhibited statistically significant growth defects relative to vector control transfectants; p-values from ANOVA analyses are shown (see S12A, S12C and S12E Fig). The insert sizes are shown in bp. Insert directionality relative to the corresponding coding sequence in the annotated genome is shown. Insert frame relative to the trigger sequence in the pTriggerAdaptor is shown. The accession numbers for coding sequences that inserts correspond to are indicated, with available annotation information and GO terms for each gene (NA, not applicable). Predicted roles in metabolic pathways are shown; genes that are predicted to function in more than one metabolic pathway are indicated as “several.” These predictions are based on data from KEGG Metabolic Pathways (https://www.kegg.jp), the MetaCyc Metabolic Pathway Database [43], and Chemical Entities of Biological Interest (ChEBI) database [44].