SH1043665.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE为界定、识别、交流以及处理基于DNA的物种假说（Species Hypotheses，SH）提供了一套统一的标准化方法。国际核酸序列数据库中的所有真菌内部转录间隔区（Internal Transcribed Spacer，ITS）序列，通过应用一套动态距离阈值（0.5%~3.0%）被聚类至近似物种水平的单元。所有物种假说（SH）均会被赋予一个以数字对象标识符（Digital Object Identifier，DOI）形式存在的唯一且稳定的标识名称，其分类学与生态学注释信息均通过分布式网络第三方注释工作完成验证。系统会尽可能结合该物种假说（SH）内所有序列的鉴定结果，将其与对应分类单元名称及其所属分类层级（如门、纲、目等）进行关联。每条物种假说（SH）均会选取一条经自动或人工指定的序列作为其代表序列。上述代表序列已通过公开链接（https://unite.ut.ee/repository.php）向全球科研社群开放，可用于本地序列相似性搜索、下一代测序（Next-Generation Sequencing，NGS）分析流程等多种科研场景。随着公共真菌ITS序列库的规模持续增长，该系统与配套数据也会自动进行更新。