Embryoid Body-based RNA-seq Analyses Reveal a Potential TBPPA Multifaceted Developmental Toxicity

In this study, we differentiated mouse ESCs via 3D aggregates called embryoid bodies in presence of environmental and human relevant TBPPA concentrations for 28 days. We collected samples at different time points and analyzed TBBPA-dependent global gene expression changes by RNA-seq. Our analyses revealed a potential TBBPA multifaceted developmental toxicity with effects on the nervous and cardiac/skeletal muscle systems. Mechanistically, our findings suggest TBBPA endocrine disrupting activities in part via prolactin signaling. Overall design: we exposed mouse EB developmental process to TBBPA and analyzed TBBPA-dependent global gene expression at different time point.