Safety assessment of Spirulina platensis through sprague dawley rats modeling

Abstract Health safety of spirulina (Spirulina platensis) for food application was evaluated through hematology and histopathology of liver, spleen and kidney in Sprague Dawley rats fed on three isoproteic diets maintained to 10% protein for forty five days. Three groups of rats were maintained as control diet casein (R-C); diet containing crude spirulina (R-S) and diet containing purified spirulina protein (R-SPI). Hematological profile of the rats group fed on spirulina diets showed no significant increase in white blood cells (WBCs), while there was decreasing trend in the levels of neutrophils (60.46 ± 2.60%) and lymphocytes (33.53 ± 1.06%) in rats that were fed on spirulina diet (R-S) than the other tested experimental diet group. Histopathological examination revealed normal architecture of the liver with no abnormality in kidneys and spleen exhibiting safety of spirulina in both forms to mitigate protein energy malnutrition widely prevalent in developing countries.

摘要：本研究通过血液学检测以及对肝脏、脾脏和肾脏的组织病理学分析，评估了钝顶螺旋藻（Spirulina platensis）用于食品的健康安全性。实验选取斯普拉格·道利大鼠（Sprague Dawley rats），为其饲喂蛋白质含量固定为10%的三种等氮日粮，持续45天。实验分为三组：对照组饲喂酪蛋白日粮（R-C组），实验组1饲喂含粗制螺旋藻的日粮（R-S组），实验组2饲喂含纯化螺旋藻蛋白的日粮（R-SPI组）。血液学检测结果显示，饲喂螺旋藻日粮的各组大鼠白细胞（white blood cells，WBCs）计数无显著升高；但相较于其余两个实验组，R-S组大鼠的中性粒细胞水平（60.46±2.60%）与淋巴细胞水平（33.53±1.06%）呈下降趋势。组织病理学检查表明，大鼠肝脏结构正常，肾脏与脾脏未出现异常，证实两种形式的螺旋藻均具备安全性，可用于缓解发展中国家广泛流行的蛋白质能量营养不良。