Bacterial microbiome of four populations of Mycopsylla fici using 16S rDNA amplicon sequencing

The goals of this experiment are to assess the bacterial diversity associated with Mycopsylla fici (Psylloidea: Homotomidae), and to compare bacterial diversity and relative abundance between populations within the native and colonised range of this species. Insects were collected on Ficus macrophylla from four locations: Brisbane, Sydney and Lord Howe Island in Australia and Auckland in New Zealand between September 2013 and December 2014. Regions v3 and v4 of the 16S rDNA were amplified using the primers 341F (5’-CCTACGGGNGGCWGCAG) and 805R (5’-GACTACHVGGGTATCTAATCC) and sequenced (paired end, 2x300bp) on a Miseq platform (Next Generation Sequencing facility, Western Sydney University). When unzipped, the folder contains 96 fastq files and one Excel file with description of the insects used in the experiment.

本实验旨在解析与Mycopsylla fici（木虱总科：扁木虱科）共生的细菌多样性，并比较该物种本土分布区与入侵定植分布区种群间的细菌多样性及相对丰度差异。实验样本于2013年9月至2014年12月间，采自澳大利亚布里斯班、悉尼、豪勋爵岛以及新西兰奥克兰共4个采样点，寄主植物为大无花果榕（Ficus macrophylla）。采用引物341F（5’-CCTACGGGNGGCWGCAG）与805R（5’-GACTACHVGGGTATCTAATCC）对16S核糖体DNA（16S rDNA）的V3、V4可变区进行扩增，并依托MiSeq测序平台（澳大利亚西悉尼大学下一代测序设施）开展双端测序，测序读长为2×300bp。解压该文件夹后，将包含96个FASTQ格式文件与1份记录实验所用昆虫样本信息的Excel表格。