Single-cell RNA-sequencing of OMP-GFP mouse olfactory sensory neurons

We sequenced the transcriptome of 34 single olfactory sensory neurons (OSNs) manually picked from OMP-GFP mice, ensuring the cells expressed high levels of GFP, a marker of mature OSNs. We pooled the olfactory mucosa from 2 male and 2 female animals, to obtain single-cell suspensions for cell picking. We only picked healthy-looking cells. From each, we constructed libraries for single-cell RNA-sequencing, to characterise the diversity of OR genes expressed.本研究对从OMP-GFP小鼠中手动挑取的34个单个嗅觉感觉神经元（olfactory sensory neurons, OSNs）的转录组进行测序，确保这些细胞高表达成熟嗅觉感觉神经元的标志物绿色荧光蛋白（GFP）。我们将2只雄性和2只雌性小鼠的嗅黏膜混合，以制备用于细胞挑取的单细胞悬液。本研究仅挑选外观状态健康的细胞，随后从每一个挑取的细胞中构建单细胞RNA测序文库，以表征所表达的嗅觉受体（olfactory receptor, OR）基因的多样性。