C-terminal regions are required for interaction.

(A) Data from quantitative LacZ assays of four overlapping regions of 200 amino acids each of PBP1 tested for interaction with the C-terminal 123 amino acids of RipA. The C-terminal 259 amino acids of PBP1 were found to be sufficient for interaction with RipA, while regions lacking the C-terminal 150 amino acids failed to interact. Data shown are from a representative experiment done in triplicate. Data are represented as mean +/− SEM. (B) Data from quantitative LacZ assays of several different deletion constructs for interaction with the C-terminal 259 amino acids of PBP1 in the yeast two-hybrid assay. Deletion of the C-terminal of RipA decreased the intensity of interaction and the C-terminal 25 amino acids of RipA were sufficient for interaction with PBP1. Data shown are from a representative experiment done in triplicate. Data are represented as mean +/− SEM.