Strand-specific Illumina TruSeq mRNA sequencing libraries of 1000 Genomes Trios

Strand-specific polyA-selected mRNA libraries were generated using Illumina TruSeq RNA Library prep kits and protocols. Targeted fragment size was 300 bp. Libraries were multiplexed, pooled, and sequenced on multiple lanes of an Illumina HiSeq2500 to an average depth > 50M paired-end 50-cycle reads for each sample. Each library also includes 1 ul of a 1:10 dilution of ERCC RNA Control Spike-Ins (Ambion) that were added from one of two mixes, each containing the same 92 synthetic RNA standards of known concentration and sequence.