CIL:43401

Wound Healing Assay Time series DIC images of mouse DA3 cells, derived from the mouse mammary adenocarcinoma cell line D1-DMBA-3, induced in BALB/C mice by dimethylbenzanthracene were grown to 90% confluence. A scratch approximately 300um wide was made in the cell monolayer, and images were recorded every 14.5 min for 26 hr. This time series image, obtained with HGF/SF treatment, is part of a group of 17 time series images (CIL:43401 to CIL:43417) representing three different treatments: (1) no HGF/SF (CIL:43406 to CIL:43411), (2) with HGF/SF (CIL:43401 to CIL:43405), activating HGF/SF-Met signaling, and (3) with PHA (Met inhibitor) and HGF/SF (CIL:43412 to CIL:43417). The group also includes two movies (CIL:43418 and CIL:43419) created from the time series CIL:43402 and CIL:43406 respectively.

本数据集为小鼠DA3细胞的划痕愈合实验（Wound Healing Assay）时间序列差分干涉对比（DIC）图像。该DA3细胞源自小鼠乳腺腺癌细胞系D1-DMBA-3，由二甲基苯并蒽（dimethylbenzanthracene）在BALB/C小鼠体内诱导获得，实验前细胞被培养至90%汇合度。研究人员在细胞单层上制作了一条宽度约300微米的划痕，随后以每14.5分钟一次的频率连续采集图像，总时长共计26小时。本数据集为经肝细胞生长因子/散射因子（hepatocyte growth factor/scatter factor, HGF/SF）处理的时间序列图像，属于包含17组时间序列图像（编号范围CIL:43401至CIL:43417）的数据集子集。该数据集涵盖三种不同处理条件：① 未添加HGF/SF对照组（CIL:43406至CIL:43411）；② HGF/SF处理组（CIL:43401至CIL:43405），该组用于激活HGF/SF-Met信号通路；③ PHA（Met抑制剂）联合HGF/SF处理组（CIL:43412至CIL:43417）。该数据集组还包含两段视频，分别由时间序列图像CIL:43402与CIL:43406生成，对应编号为CIL:43418和CIL:43419。