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资源简介：
Leveraging transposable elements of the genome to prevent Cas9 editing and design a safety-switch for cell therapies
应用场景：
创建时间：
2020-01-31
相关数据集
HDR-CRISPR promotes targeted genome editing with reduced mutational burden in primary human cells [CAST-seq]. HDR-CRISPR promotes targeted genome editing with reduced mutational burden in primary human cells [CAST-seq]
We have established a technology to endorse DSB resolution via HDR with the goal of facilitating clinical transition of HDR-based editing strategies and increase safety. We have used high throughput s
NIAID Data Ecosystem70
Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases
We describe a robust linear amplification-mediated high-throughput genome-wide translocation sequencing (HTGTS) method that identifies endogenous or ectopic "prey" DNA double-stranded breaks (DSBs) ac
NIAID Data Ecosystem70
Antizyme protects against abnormal accumulation and toxicity of polyamines in ornithine decarboxylase-overproducing cells.
Exposure of ornithine decarboxylase (ODC; L-ornithine carboxy-lyase, EC 4.1.1.17)-overproducing mouse FM3A cells to micromolar levels of spermine or spermidine caused abnormal accumulation and toxicit
PubMed Central1994-09-13 更新40
Quantitative evaluation of chromosomal rearrangements in primary gene-edited human stem cells by preclinical CAST-Seq. Quantitative evaluation of chromosomal rearrangements in primary gene-edited human stem cells by preclinical CAST-Seq
Genome editing with programmable nucleases has shown great promise for clinical translation but also revealed the risk of genotoxicity caused by chromosomal translocations or the insertion of mutation
NIAID Data Ecosystem70
No observable guide-RNA-independent off-target mutation induced by prime editor [RNA-Seq]
Prime editor (PE) has been recently developed to induce efficient and precise on-target editing, whereas its guide RNA (gRNA)-independent off-target effects remain unknown. Here, we used whole-genome
NIAID Data Ecosystem50
