Phenotypic analysis of resident peritoneal MØ subsets.
收藏Figshare2015-12-02 更新2026-04-29 收录
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SPM and LPM were gated as shown in Figure 1 and the median fluorescence intensity (MFI) of F4/80, CD11b, IAb, CD80, CD86, CD40, LY6C, DC-SIGN, Dectin-1 and TLR4 was analyzed by flow cytometry. All acquisitions were performed using a 5-color staining combination. The value for the expression of each surface marker was obtained by subtracting the MFI of the fluorescence-minus-one control (FMO) from the MFI of the whole stained cell population [23], [25], as described in the Materials and Methods (M&M) section. Data are representative of more than 3 independent experiments.
创建时间：
2015-12-02
