RNA-sequencing (RNA-Seq) analysis.xlsx

HIV-1 possesses the capacity to exploit the metabolic resources of host cells. To better understand the metabolic abnormalities associated with HIV-1, RNA-sequencing was applied. The analysis was performed using 3 biological replicates in HIV-1 infected macrophages group and control group (N = 6) . The total RNA was extracted using TRIzol reagent (Sigma-Aldrich, USA) following the manufacturer’s procedure.

人类免疫缺陷病毒1型（HIV-1）具备利用宿主细胞代谢资源的能力。为深入解析与HIV-1感染相关的代谢异常，本研究采用了RNA测序（RNA-sequencing）技术。本分析针对HIV-1感染巨噬细胞组与对照组各设置3次生物学重复，总样本量N=6。总RNA提取采用TRIzol试剂（Sigma-Aldrich，美国），并严格遵循产品制造商的操作流程。