Genetic surexpression of human BIN1 as a therapeutic approach in a murine model of X-linked myotubular myopathy (Mtm1-/y)

In this study, we performed RNA sequencing on tibialis anterior muscle of male mice at 7 weeks of age. WT and Mtm1 deficient (Mtm1-/y) mice were sequenced. The rescued mice, Mtm1-/y mice crossed with TgBIN1 mice (Mmt1-/yTgBIN1) and the associated control mice TgBIN1 were also sequenced. The study is composed of 4 groups of mice: WT, Mtm1-/y, TgBIN1 and Mtm1-/yTgBIN1. Each group was composed from triplicates to quadruplicates. The comparison of transcriptome profiles across the different genotypes was assessed in this study.本研究对7周龄雄性小鼠的胫骨前肌（tibialis anterior muscle）开展了RNA测序（RNA Sequencing）。本研究对野生型（Wild Type，WT）及Mtm1缺陷型（Mtm1-/y）小鼠进行了测序；同时还对拯救型小鼠——即Mtm1-/y小鼠与TgBIN1小鼠杂交获得的Mtm1-/yTgBIN1小鼠——及其对应的对照小鼠TgBIN1进行了测序。本研究共设置4组小鼠：WT、Mtm1-/y、TgBIN1及Mtm1-/yTgBIN1，每组包含3~4个生物学重复。本研究旨在分析不同基因型小鼠的转录组（transcriptome）表达谱差异。