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Human Clariom S Array in OVCAR8 cells after silencing HSD17B14

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NIAID Data Ecosystem2026-05-01 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE173253
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We used a functional small interfering RNA (siRNA) screen in ovarian cancer cells to identify 17β-hydroxysteroid dehydrogenase type 14 (HSD17B14) as a novel regulator of small EV secretion. We showed that the knockdown of HSD17B14 altered multiple gene expression in OVCAR8 cells. OVCAR8 cells were transfected with siControl and siHD17B14 for 48 hours. Total RNA was isolated using TRizol reagent (Thermo Fisher Scientific) and Direct-zol column kit (Genesee Scientific).
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2024-04-23
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