Gut microbiota analysis associated with autism spectrum disorders [16S rRNA]

Here we report 16S rRNA data in gut microbiota of autism spectrum disorders compared with healthy volunteers. A total of 1322 operational taxonomic units (OTUs) were identified in the sequence data. The Bacteroidetes and Firmicutes were both dominated phylum in ausitic subjects and healthy controls. Phylum level analysis showed a clear alteration of the bacterial gut community in ASD characterized by a higher Firmicutes (P < 0.05), Proteobacteria (P < 0.001), and Actinobacteria (P < 0.001) than that in healthy controls. However, Bacteroidetes were significantly decreased in ASD patients (P < 0.001). Overall design: Microbial DNA from fecal samples [healthy control (n=143), ASD patients (n=143)] were amplified by PCR using universal bacterial primers. PCR was performed to produce V4 regions of the 16S rDNA gene using the conserved primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). 16S rRNA were sequenced by Novogene Bioinformatics Technology Co., Ltd. (Shanghai, China). Subject IDs (e.g. A3) in this study represent the same individual with identical id in GSE113540.