Albumosomes formed by pre-folding albumin in cytoplasm of hepatocytes maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease

Wildtype (WT) and albumin-knockout (AKO) were fed with normal diet. We harvested the liver tissues from WT and AKO mice at 3-week, 8-week, and 10-month of age. We then performed gene expression profiling analysis using data obtained from RNA-seq of the six different liver tissues. Wildtype (WT) and albumin-knockout (AKO) mice were fed with Normal diet (ND) or Western diet (WD) for 8 weeks from the age of 8 weeks. We harvested the liver tissues from WT-ND, WT-WD, AKO-ND, AKO-WD mice . We then performed gene expression profiling analysis using data obtained from RNA-seq of the four different liver tissues. Wildtype (WT) mice fed with HFD from the age of 8 weeks were injected by 17-AAG at the 3rd, 4th, 5th, 6.5th, and 8th weeks of the HFD feeding process. The control group was injected by the solvent. Another group of mice at the same age was fed with normal diet (ND). We harvested the liver tissues ND, Ctrl, and 17-AAG mice. We then performed gene expression profiling analysis using data obtained from RNA-seq of the three different liver tissues.将野生型（Wildtype，WT）与白蛋白基因敲除（albumin-knockout，AKO）小鼠饲喂正常饮食，分别于3周龄、8周龄及10月龄时采集其肝脏组织，随后利用6份不同肝脏组织样本的RNA测序（RNA-seq）数据开展基因表达谱分析。将8周龄的WT与AKO小鼠分别饲喂正常饮食（Normal diet，ND）或西式饮食（Western diet，WD），持续干预8周；采集WT-ND、WT-WD、AKO-ND及AKO-WD各组小鼠的肝脏组织，利用4份不同肝脏组织样本的RNA-seq数据进行基因表达谱分析。对8周龄起饲喂高脂饮食（High Fat Diet，HFD）的WT小鼠，于高脂饲料喂养的第3、4、5、6.5及第8周注射17-AAG；对照组注射等量溶剂。另设同龄饲喂正常饮食（ND）的小鼠作为额外对照，采集ND组、溶剂对照组（Ctrl）及17-AAG处理组小鼠的肝脏组织，利用3份不同肝脏组织样本的RNA-seq数据开展基因表达谱分析。