Performance of five food regimes on Anopheles gambiae senso stricto larval rearing to adult emergence in Insectary

Background: Rearing of Anopheles gambiae s.s mosquitoes in insectary with quality cheap food sources is of paramount importance for better and healthy colony. This study evaluated larval survival and the development rate of aquatic stages of An.gambiae s.s under five food regimes; tetramin fish food (a standard insectary larval food), maize pollen, Cerelac, green filamentous algae and dry powdered filamentous algae. Methods: Food materials were obtained from different sources, cerelac was made locally, fresh filamentous algae was taken from water bodies, dry filamentous algae was ground to powder after it was dried under shade, and maize pollen was collected from the flowering maize. Each food source type was used to feed three densities of mosquito larvae 20, 60, and 100 in six replicates each. Larval age structure was monitored daily until pupation and subsequently adult emergence. Tetramin was used and taken as a standard food source for An. gambiae s.s. larvae feeding in Insectary. ...

背景：在昆虫饲养室中使用优质廉价的食源饲养冈比亚按蚊（Anopheles gambiae s.s.），对于培育优质健康的蚊群至关重要。本研究针对五种食源条件下冈比亚按蚊水生阶段的幼虫存活率与发育速率展开评估，五种食源分别为：Tetramin鱼粮（昆虫饲养室标准幼虫饲料）、玉米花粉、Cerelac婴儿米粉、绿色丝状藻类以及干燥粉末状丝状藻类。方法：本次实验的饲料原料取自不同渠道：Cerelac为本地自制，新鲜丝状藻类采自自然水体，干燥丝状藻类经阴干后研磨为粉末，玉米花粉则采集自开花期的玉米植株。每种饲料分别用于饲喂三个密度的蚊幼虫（20、60、100只/组），每组设置6次生物学重复。每日监测幼虫龄期结构，直至化蛹及后续成虫羽化。本研究以Tetramin鱼粮作为昆虫饲养室中冈比亚按蚊幼虫饲养的标准对照饲料。……