Comparison of cerebellum between High Runner and Control mouse genotypes in Free or Blocked from Wheel running environments

The goals of this study were: 1) to compare the cerebellum transcriptome and associated networks of one of the High Runner mouse lines relative to mice from a control line and, 2) to evaluate the modulation of potential transcriptome differences between genotypes by the environmental opportunity for physical activity. At approximately 6 weeks of age, 8 mice from a High Runner line and 8 mice from a Control line were caged individually with free access to wheel for 7 days. On day 7, half of the mice from each genotype were blocked from entering the running wheel. Samples were profiled using paired-end RNA sequencing本研究的目标如下：1）对比其中一种高奔跑能力小鼠品系（High Runner mouse line）与对照组品系（control line）小鼠的小脑转录组（cerebellum transcriptome）及其相关基因调控网络；2）评估身体活动的环境机会对不同基因型小鼠间潜在转录组差异的调控效应。 在小鼠约6周龄时，将8只高奔跑品系小鼠与8只对照组品系小鼠单独笼养，使其可自由使用跑轮，饲养周期为7天。 实验第7天时，限制每个基因型组中半数小鼠使用跑轮。 所有样本均采用双端RNA测序（paired-end RNA sequencing）技术进行转录组分析。