FASTQ files associated with two mPAT experiments

The mPAT appraoch (Beilharz et al 2017; Genetics) was used to quantitate mRNA expression and poly (A)-tail length distribution changes in the early fly embryo by amplicon sequencing."fastq-files.tar.gz" contains short reads from two experiments following the mPAT protocol, in FASTQ format. The reads have been subsetted to include only reads relating to the nos, pgc, and tim10 genes. Primers used to amplify these genes are listed in "primers.csv", excluding the adaptor sequence component of these primers. Read counts and Reads Per Million for these genes are listed in "mPAT-reads.csv".

mPAT方法（Beilharz等，2017年；遗传学）被应用于对果蝇早期胚胎中mRNA表达以及多聚（A）-尾长度分布变化进行量化。'fastq-files.tar.gz'文件包含了遵循mPAT协议进行的两个实验的短读段，格式为FASTQ。经过筛选，仅包含与nos、pgc和tim10基因相关的读段。用于扩增这些基因的引物列表详见'primers.csv'文件，其中已排除引物中的适配器序列部分。这些基因的读段计数和每百万读段数均在'mPAT-reads.csv'文件中列出。