Hemileia vastatrix isolate:Race XXXIII Genome sequencing and assembly. Hemileia vastatrix isolate:Race XXXIII

Coffee leaf rust caused by the fungus Hemileia vastatrix is one of the most important leaf diseases affecting coffee plantations worldwide. Current knowledge of the H. vastatrix genome is limited and only a small fraction of the total fungal secretome has been identified. In order to obtain a more comprehensive understanding of the fungus secretome, the main goal of this study was to sequence and assemble the entire genome of H. vastatrix urediniospores race XXXIII using two next-generation sequencing platforms with long (PacBio) and short (Illumina) reads and a hybrid assembly strategy for achieving a high quality reference genome.We used in silico methods to predict secreted proteins, and a subset of their corresponding genes were subject to time course-based, gene expression studies. Together, our data provide the most comprehensively sequenced and assembled coffee rust genome to date and functional data on the putative secretome.