RNA-SEQ of Rpd3 and WT in Aspergillus flavus

To investigate the genes regulated by Rpd3 in these biological functions, we performed RNA-seq analysis on the WT and Rpd3 mutant strains of A. flavus. The quality control results demonstrate excellent parallelism between samples. Our findings indicate that the deletion of Rpd3 results in the upregulation of 583 genes and the downregulation of 392 genes compared to the WT, underscoring its significant influence on transcriptional regulation . Gene Set Enrichment Analysis (GSEA) revealed that the deletion of Rpd3 leads to the downregulation of processes related to redox reactions . We further assessed the levels of ROS in the mycelia., Rpd3 strains accumulated higher ROS levels and hydrogen peroxide.Subcellular localization of Rpd3