DNA metabarcoding of soil eDNA samples for monitoring plant diversity in the Amazon canga of Serra dos Carajás (Pará, Brazil).

DNA metabarcoding as a complementary approach to traditional surveys for monitoring plant diversity in ironstone outcrops (canga) of the Serra dos Carajás in the Eastern Amazon (Pará, Brazil). This study demonstrates the application of DNA metabarcoding of soil eDNA for surveying plant diversity in the cangas of Serra dos Carajás, to support monitoring efforts and biodiversity assessments in this unique and diverse Amazon ecosystem. Soil eDNA samples were collected from four plots (PCG-168, PCG-121, PCG-173, and PCG-235) during the dry season. The nuclear ITS2 region was amplified and sequenced using the Illumina MiSeq platform. This project is part of the Canga Plant Genomics initiative (Bioproject PRJNA448777), which focuses on genomic technologies and bioinformatics for studying plants in canga ecosystems.[This dataset was processed using the GBIF Metabarcoding Data Toolkit.]

DNA宏条形码（DNA metabarcoding）作为传统调查的补充方法，用于监测亚马逊东部（巴西帕拉州）卡拉加斯山脉铁质岩露头（canga）的植物多样性。本研究展示了土壤环境DNA（eDNA）的DNA宏条形码技术在卡拉加斯山脉canga区域开展植物多样性调查的应用，以支撑该独特多样的亚马逊生态系统的监测工作与生物多样性评估。研究于旱季从4个样地（PCG-168、PCG-121、PCG-173及PCG-235）采集了土壤eDNA样本。采用Illumina MiSeq测序平台对核基因组ITS2区域进行扩增与测序。本项目隶属于Canga植物基因组学倡议（Bioproject PRJNA448777），该倡议聚焦于利用基因组技术与生物信息学方法研究canga生态系统中的植物。本数据集通过GBIF宏条形码数据工具包（GBIF Metabarcoding Data Toolkit）完成处理。