Next-generation sequencing facilitates quantitative analysis of atrial and ventricular cardiomyocytes transcriptomes

To investigate the chamber selective transcriptional programs, we performed RNA-seq on purified cardiomyocytes. Totally we have 5 replicates for atrial cardiomyocytes and 5 replicates for ventricular cardiomyocytes. Comparative gene expression profiling analysis of RNA-seq data for atrial cardiomyocytes and ventricular cardiomyocytes.为探究心脏腔室选择性转录程序，我们对纯化的心肌细胞开展了RNA测序（RNA-seq）实验。本研究中共设置5组心房心肌细胞生物学重复与5组心室心肌细胞生物学重复，并针对两类心肌细胞的RNA-seq数据开展了比较基因表达谱分析。