Bacterial 16s rRNA gene abundance based on quantitative PCR data from incubations with hydrocarbons

DNA-based stable-isotope probing with uniformly (13)C-labelled hydrocarbons followed by DNA sequencing was used to identify oil degrading bacteria present in surface and oil plume water associated with the Deepwater Horizon blowout. Further, qPCR specific to the groups identified as being involved in oil degradation via DNA-SIP was performed to show these groups respond to oil addition with increased abundance. This dataset includes 16S rRNA gene abundances acquired via qPCR. Data were generated through laboratory incubations of surface water collected near 28° 4.175N 88°22.335 W on May 5th 2010 and within plume water collected on May 31st 2010 at 28° 41.686 N 88° 26.081 W. The dataset listed under this UDI: R1.x132.135:0001 - is exclusive to Hydrocarbon-degrading bacterial DNA sequence and abundance.

本研究采用DNA稳定同位素探针技术（DNA-based stable-isotope probing, DNA-SIP）结合均匀¹³C标记烃类物质，随后开展DNA测序，以识别深水地平线油井喷事故相关的表层海水与油羽流水中存在的石油降解细菌。进一步，针对通过DNA-SIP鉴定出的参与石油降解的细菌类群，实施了特异性定量PCR（quantitative PCR, qPCR）实验，结果证实此类群在添加石油后丰度显著升高。本数据集包含通过qPCR获取的16S rRNA基因丰度数据。相关数据来源于2010年5月5日采集自北纬28° 4.175′、西经88°22.335′附近的表层海水，以及2010年5月31日采集自北纬28° 41.686′、西经88° 26.081′的油羽流海水的实验室培养实验。本数据集在唯一设备标识符（unique device identifier, UDI）R1.x132.135:0001下所列出的内容，仅包含烃类降解细菌的DNA序列及其丰度信息。