RNA-seq analysis evaluating effects of Adssl1 overexpression in neonatal rat ventricular myocytes (NRVM)

Reactivating cell cycle re-entry in adult cardiomyocytes improves cardiac function after myocardial infarction (MI). De novo purine synthesis is a critical source of nucleotides and is involved in modulating cell proliferation. Here, using gain-of-function genetic tools, we explored the role of the de novo purine synthesis enzyme Adssl1 in cardiac regeneration. RNA sequencing analysis suggested that Adssl1 overexpression induced strong dedifferentiation and cell cycle entry.激活成体心肌细胞的细胞周期再进入，可改善心肌梗死（MI）后的心功能。从头嘌呤合成（de novo purine synthesis）是核苷酸的关键来源，并参与调控细胞增殖。本研究采用功能获得性遗传工具，探究了从头嘌呤合成酶Adssl1在心脏再生中的作用。RNA测序（RNA sequencing）分析表明，Adssl1过表达可诱导显著的细胞去分化与细胞周期再进入。