Data from: The adsorption features between insecticidal crystal protein and Nano-Mg(OH)2

Nano-Mg(OH)2, with low biological toxicity, is an ideal nano-carrier for insecticidal protein to improve the bioactivity. In this work, the adsorption features of insecticidal protein by nano-Mg(OH)2 have been studied. The adsorption capacity could achieve as high as 136 mg/g, and the adsorption isotherm had been fitted with Freundlich model. Moreover, the adsorption kinetics followed pseudo-first or -second order rate model, and the adsorption was spontaneous and exothermic process. However, the high temperatures are not suitable to adsorption, which implies that the temperature would be a critical factor during the adsorption process. In addition, FT-IR confirmed that the protein was adsorbed on the nano-Mg(OH)2, the Zeta potential analysis suggested that insecticidal protein was loaded onto the nano-Mg(OH)2 not by electrostatic adsorption but might by intermolecular forces, and the circular dichroism (CD) spectroscopy of Cry11Aa protein before and after loading with nano-Mg(OH)2 was changed. The study applied the adsorption information between Cry11Aa and nano-Mg(OH)2, which would be useful in the real appilation of nano-Mg(OH)2 as a nano-carrier.

低生物毒性的纳米氢氧化镁（Nano-Mg(OH)₂）是一类理想的杀虫蛋白纳米载体，可有效提升杀虫蛋白的生物活性。本研究针对纳米氢氧化镁对杀虫蛋白的吸附特性展开了系统探究。实验结果显示，其吸附容量最高可达136 mg/g，且吸附等温线符合Freundlich模型拟合规律。此外，吸附动力学符合准一级或准二级速率模型，该吸附过程为自发放热反应，但高温环境不利于吸附进行，表明温度是吸附过程中的关键影响因素。傅里叶变换红外光谱（FT-IR）证实杀虫蛋白已吸附于纳米氢氧化镁表面；Zeta电位（Zeta potential）分析表明，杀虫蛋白并非通过静电吸附结合至纳米氢氧化镁，而是可能依靠分子间作用力完成负载；负载前后Cry11Aa蛋白的圆二色性（CD）光谱发生了显著变化。本研究获取的Cry11Aa蛋白与纳米氢氧化镁间的吸附特性数据，可为纳米氢氧化镁作为杀虫蛋白纳米载体的实际应用提供重要理论支撑。