Interleukin-8 is increased in chronic kidney disease in children, but not related to cardiovascular disease

Abstract Introduction: In this study, we aimed to detect the cytokine that is involved in the early stage of chronic kidney disease and associated with cardiovascular disease. Methods: We included 50 patients who were diagnosed with predialytic chronic kidney disease and 30 healthy pediatric patients in Ege University Medical Faculty Pediatric Clinic, İzmir/Turkey. Interleukin-8 (IL-8), interleukin-10 (IL-10), interleukin-13 (IL-13), and transforming grow factor-β1 (TGF-β1) levels (pg/mL) were measured by ELISA. Carotid-femoral pulse wave velocity (PWV), augmentation index (Aix), carotid intima media thickness (cIMT), and left ventricular mass index (LVMI) were evaluated as markers of cardiovascular disease. The presence of a cardiovascular disease marker was defined as an abnormality in any of the parameters (cIMT, PWV, Aix, and left ventricular mass index (SVKI)). The patient group was divided into two groups as with and without cardiovascular disease. Results: Mean Aix and PWV values were higher in CKD patients than controls (Aix: CKD 32.8±11.11%, healthy subjects: 6.74±6.58%, PWV CKD: 7.31±4.34m/s, healthy subjects: 3.42±3.01m/s, respectively; p=0.02, p=0.03). The serum IL-8 levels of CKD were significantly higher than of healthy subjects 568.48±487.35pg/mL, 33.67±47.47pg/mL, respectively (p<0.001). There was no statistically significant difference between IL-8, IL-10, IL-13, TGF-1, in CKD patients with and without cardiovascular disease (p> 0.05). Discussion: IL-8 is the sole cytokine that increases in pediatric patients with chronic kidney disease among other cytokines (IL-10, IL-13 and TGF-β1). However, we did not show that IL-8 is related to the presence of cardiovascular disease.

摘要：本研究旨在检测参与慢性肾脏病早期进程且与心血管疾病相关的细胞因子。方法：本研究纳入土耳其伊兹密尔市埃格大学医学院儿科诊所收治的50例透析前慢性肾脏病患者，以及30名健康儿科受试者。采用酶联免疫吸附试验（ELISA）检测白细胞介素-8（Interleukin-8, IL-8）、白细胞介素-10（Interleukin-10, IL-10）、白细胞介素-13（Interleukin-13, IL-13）及转化生长因子-β1（Transforming Growth Factor-β1, TGF-β1）的血清水平（单位：pg/mL）。以颈动脉-股动脉脉搏波速度（Carotid-Femoral Pulse Wave Velocity, PWV）、增强指数（Augmentation Index, Aix）、颈动脉内膜中层厚度（Carotid Intima Media Thickness, cIMT）及左心室质量指数（Left Ventricular Mass Index, LVMI）作为心血管疾病相关标志物。心血管疾病标志物阳性定义为任意一项上述参数（cIMT、PWV、Aix及左心室质量指数）出现异常。将患者组分为合并心血管疾病组与未合并心血管疾病组。结果：慢性肾脏病患者的平均Aix及PWV值均高于健康对照组（Aix：慢性肾脏病组32.8±11.11%，健康对照组6.74±6.58%；PWV：慢性肾脏病组7.31±4.34m/s，健康对照组3.42±3.01m/s；两组比较分别为p=0.02、p=0.03）。慢性肾脏病患者的血清IL-8水平显著高于健康对照组，分别为568.48±487.35pg/mL与33.67±47.47pg/mL（p<0.001）。合并与未合并心血管疾病的慢性肾脏病患者之间，IL-8、IL-10、IL-13及TGF-β1的水平差异均无统计学意义（p>0.05）。讨论：在IL-10、IL-13与TGF-β1等细胞因子中，IL-8是儿科慢性肾脏病患者中唯一出现水平升高的细胞因子。但本研究未证实IL-8与心血管疾病的发生存在关联。