Partial gene catalog of deep samples (4000m) from a Global Metagenomics Expedition (Malaspina 2010)

The phylogenetic composition of the heterotrophic microbial community is depth stratified in the oceanic water column down to abyssopelagic layers. In the layers below the euphotic zone, it has been suggested that heterotrophic microbes rely largely on solubilized particulate organic matter as a carbon and energy source rather than on dissolved organic matter. To decipher whether changes in the phylogenetic composition with depth are reflected in changes in the bacterial and archaeal transporter proteins, we generated an extensive metaproteomic and metagenomic dataset of microbial communities collected from 100- to 5,000-m depth in the Atlantic Ocean.

异养微生物群落的系统发育组成在海洋水柱中呈深度分层格局，直至深渊层。在真光层下方的水层中，已有研究表明异养微生物主要以可溶性颗粒有机质作为碳源与能量来源，而非溶解有机质。为阐明随深度变化的系统发育组成是否与细菌和古菌转运蛋白的变化相对应，我们对大西洋100米至5000米水深采集的微生物群落构建了大规模宏蛋白质组（metaproteomic）与宏基因组（metagenomic）数据集。