MOESM4 of Deletion of the 2-acyl-glycerophosphoethanolamine cycle improve glucose metabolism in Escherichia coli strains employed for overproduction of aromatic compounds
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Additional file 4: Table S3. RT-qPCR mRNA expression values of central carbon metabolism, in the evolved strains. Relative mRNA concentrations of central metabolism and regulatory genes of PB11, PB12 and PB13 strains, grown in glucose as the sole carbon source were determined by RT-qPCR. Most of these values have been previously reported (*) and are presented here for discussion and comparison purposes [2, 9–11]. Data in this table are reported as relative expression levels of the parental strain JM101. The mRNA level of each gene in the parental strain was used as control to normalize the data, assigning it the value of one. N.D. Not determined.
附加文件4:表S3。进化菌株中心碳代谢相关基因的实时荧光定量反转录PCR(RT-qPCR)mRNA表达量。本研究通过RT-qPCR技术,测定了以葡萄糖为唯一碳源培养的PB11、PB12及PB13菌株的中心代谢与调控基因的相对mRNA丰度。其中多数数据此前已发表(*),本次收录以供讨论与对比参考[2, 9–11]。本表数据以亲本菌株JM101的相对表达水平为基准呈现。以亲本菌株各基因的mRNA水平作为对照对数据进行归一化处理,并将该对照值赋值为1。N.D. 表示未测定(Not determined)。
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Figshare
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2017-12-19



