Photosynthetic primary production of microplankton combined with different size fractions in sea water from the euphotic zone of the Aegean Sea at Station MARCH-1997-GN36199704601MSB01

Primary production was measured with the in situ 14C method of Steeman-Nielsen (Steemann-Nielsen, E. 1952. The use of radio-active carbon 14C for measuring organic production in the sea. Journal du Conseil International pour l'Exploration de la Mer. 18:3, 117-140). Samples were dispensed in 250 ml transparent polycarbonate bottles (three light and one dark for each depth); and each one was inoculated with 5 µCi 14-C-NaHCO3 and incubated at the collection depths for 2 hours. Fractionation was carried out by filtration through 0.2 µm, 1.2 µm and 3.0 µm polycarbonate Millipore filters. Filters were placed in scintillation vials, acidified with 0.5 N HCl and counted in a liquid scintillation counter.

本研究采用Steeman-Nielsen提出的原位14C法（in situ 14C method）（Steemann-Nielsen, E. 1952. 放射性碳14C在海洋有机生产力测量中的应用. 国际海洋考察理事会期刊. 18:3, 117-140）对初级生产力进行测定。实验样品分装于250mL透明聚碳酸酯瓶中，每个采样深度设置3个光照组与1个黑暗组；向每个瓶中接种5微居里的14C-碳酸氢钠（14C-NaHCO3），并在原采样深度下培养2小时。后续通过0.2μm、1.2μm及3.0μm孔径的密理博（Millipore）聚碳酸酯滤膜进行分级过滤。将滤膜置于闪烁瓶中，用0.5N盐酸酸化后，利用液体闪烁计数器完成放射性计数。