Synaptic cell adhesion molecule Cdh6 identifies a class of sensory neurons with novel functions in colonic motility

Intrinsic sensory neurons are an essential part of the enteric nervous system (ENS) and play a crucial role in gastrointestinal tract motility and digestion. Neuronal subtypes in the ENS have been distinguished by their electrophysiological properties, morphology, and expression of characteristic markers, notably neurotransmitters and neuropeptides. Here, we investigated synaptic cell adhesion molecules as novel cell type markers in the ENS. Our work identifies two Type II classic cadherins, Cdh6 and Cdh8, specific to sensory neurons in the mouse colon. We show that Cdh6+ neurons demonstrate all other distinguishing classifications of enteric sensory neurons, including marker expression of Calcb and Nmu, Dogiel type II morphology and AH-type electrophysiology and IH current. Optogenetic activation of Cdh6+ sensory neurons in the distal colon evokes retrograde colonic motor complexes (CMCs), while pharmacologic blockade of ..., Electrophysiological recordings:
Whole cell patch clamp electrophysiological recordings of Hb9:GFP+ neurons were performed according to Osorio & Delmas (14), with modifications for recording from the distal colon. The protocol is described in brief below.
Tissue dissection and preparation:
Mice aged 8–10 weeks were culled by CO2 and cervical dislocation. The colon was removed and flushed with ice cold oxygenated Krebs solution [118 mM NaCl, 4.8 mM KCl, 1 mM NaH2PO4, 25 mM NaHCO3, 1.2 mM MgCl2, 2.5 mM CaCl2 and 11 mM glucose, supplemented with scopolamine (2 M) and nicardipine (6 μM)], then placed in a Sylgard-lined Petri dish with ice cold oxygenated Krebs solution for further dissection. Krebs' solution was changed out for a fresh oxygenated solution every 5 minutes. Under a dissection microscope, the distal colon was pinned, and the mucosa was peeled away using fine forceps, leaving a few millimeters of mucosa along the edges of the tissue for pinning stability. The musculari..., , # Data from: Synaptic cell adhesion molecule *Cdh6* identifies a class of sensory neurons with novel functions in colonic motility

[https://doi.org/10.5061/dryad.66t1g1kbt](https://doi.org/10.5061/dryad.66t1g1kbt)

## Description of the data and file structure

Slice whole cell recording using pClamp of hb9-GFP cells. Each experiments start with the cell name ( letter followed by date code). An Excel with metadata info has been made (SummaryEphysData_hb9). Only non-leaky cells were kept to continue the experiment. Only the cells with a good access resistance were kept for the analysis. 

### Files and variables

#### File: A210921_0000.abf

**Description:** Membrane test protocol to evaluate access resistance, input resistance, and capacitance.

#### File: A210921_0002.abf

**Description:** Current clamp protocol to evaluate spiking property

#### File: A210921_0003.abf

**Description:** Current clamp protocol to evaluate spiking property- test 

#### File: A220509_0002.abf

**Descript..., ,