Lactic Bacterium and Bacillus Sp. Biofilms Can Decrease the Viability of Salmonella Gallinarum, Salmonella Heidelberg, Campylobacter Jejuni and Methicillin Resistant Staphylococcus Aureus on Different Substrates

ABSTRACT This study aimed to evaluate the efficacy of biofilms formed by lactic acid bacteria and Bacillus sp. (BLA) in preventing and controlling the formation of wild biofilms and/or planktonic forms of Salmonella Gallinarum (SG), Salmonella Heidelberg (SH), and methicillin-resistant Staphylococcus aureus (MRSA) on different surfaces. The SH and SG viability was evaluated in polystyrene plates, wood shavings, and soil samples. Two protocols were developed to examine the use of BLA in a preventive and control application. For analysis of Campylobacter jejuni (CJ) BLA was used only preventively in a polystyrene plate. Results showed that BLA was effective in preventing the growth of SG and SH in all matrices. The effectiveness of BLA for MRSA was lower than for SG and SH. The efficiency of BLA in preventing CJ growth seems to be related to the initial CJ contamination. BLA proves to be a potential alternative to control food-borne pathogens commonly encountered in animal production and food industry.

摘要 本研究旨在评估乳酸菌与芽孢杆菌属（Bacillus sp.，简称BLA）形成的生物被膜，在多种载体表面预防和控制野生生物被膜及/或鸡沙门氏菌（Salmonella Gallinarum，简称SG）、海德堡沙门氏菌（Salmonella Heidelberg，简称SH）与耐甲氧西林金黄色葡萄球菌（MRSA）的浮游态菌体形成的效果。研究针对聚苯乙烯培养板、木屑与土壤样本中的SH和SG存活情况开展了检测。本研究开发了两种实验方案，分别用于考察BLA的预防性应用与防控性应用效果。针对空肠弯曲菌（Campylobacter jejuni，简称CJ）的相关检测中，BLA仅在聚苯乙烯培养板中以预防性方式施用。实验结果显示，BLA可在所有实验载体中有效抑制SG与SH的增殖。BLA对MRSA的防控效果弱于其对SG和SH的作用效果。BLA抑制CJ增殖的效率似乎与CJ的初始污染量相关。综上，BLA可作为一种潜在防控手段，用于防治动物生产与食品工业中常见的食源性致病菌。