Supplemental data to "Estimating the Genetic Diversity of Pacific salmon and trout using Multi-gene eDNA Metabarcoding"

This dataset contains DNA sequence data of Oncorhynchus species, isolated from environmental DNA (eDNA) from Pacific Northwest streams via microfluidic eDNA metabarcoding and high-throughput (Ilummina) sequencing (samples collected from 2017-09-22 to 2017-10-10). It is accompanied by scripts and commands for data analyses including: sequence denoising, calculation of entropy values by codon position, and calculation of diversity statistics and haplotype mapping. Intermediate outputs include denoised haplotypes, entropy calculations, and haplotype summaries following chimera removal.

本数据集涵盖大麻哈鱼属（Oncorhynchus）的DNA序列数据，这些序列通过微流控环境DNA（eDNA）元条形码技术结合高通量（Illumina）测序，从太平洋西北地区溪流的环境DNA样本中分离获得，样本采集周期为2017年9月22日至2017年10月10日。本数据集附带配套的数据分析脚本与命令，分析内容包括：序列去噪、基于密码子位置的熵值计算、多样性统计量测算以及单倍型映射。其中间产出物包含经嵌合体去除后的去噪单倍型、熵值计算结果，以及单倍型汇总统计文件。