Region specific astrocyte response to mutant GFAP in Alexander disease model mice during postnatal development.

Gene expression analysis was performed in Gfap+/+, Gfap+/R236H, and mGFAPTg170-2 transgenic mice, using the Aldh1l1-eGFP-L10a transgenic line (JD130) to isolate translating ribosomes and purify astrocyte specific transcripts for RNAseq. Hippocampus and corpus callosum were collected from male mice at P14 in an FVBB6F1 background. Translating ribosomes were isolated using antibodies against EGFP to precipitate the EGFP-L10a fusion protein and the associated RNA purified for sequencing and gene expression analysis. N = 3 for each genotype with each sample consisting of tissues pooled from 5 mice (15 mice total).