青藏高原阿木错、班戈错、帕隆4号冰川、玉珠峰冰芯可培养细菌菌落照片（2019）

该数据集包括青藏高原阿木错（AMC）、班戈错(BGC)、帕隆4号冰川(PL4)、玉珠峰冰芯(YZF)的可培养细菌菌落图片，可以体现菌落的颜色、透明度等基本信息。附有一个表格信息，包括菌落来源地的经纬度，水深及颜色等。
这些细菌从青藏高原冰雪、湖水等生境中分离得到，菌落表现出不同的颜色，这可能与细菌适应青藏高原强辐射、低温、寡营养的环境有关，通过不同的色素产能等来适应恶劣环境。菌落是通过湖水平板涂布，在低温（4℃）培养（培养基为R2A等）分离，确定无污染、为单菌落之后获得。得到的单菌落后续会进行16S rRNA全长测序，通过97%的相似度确定其是否为新的种；如果该序列与最相似菌株的序列相似度低于97%，会进行全基因组测区，确定该菌株在相应的属、科甚至门类中的系统发育关系，确定该菌株是否具有新的发育地位，补充青藏高原湖泊微生物多样性。

This dataset contains images of cultivable bacterial colonies collected from four sampling sites on the Qinghai-Tibet Plateau: Amu Co (AMC), Bangga Co (BGC), Palong No.4 Glacier (PL4), and the Yuzhu Peak ice core (YZF). These images showcase basic morphological traits of the colonies, including color and transparency. The dataset is accompanied by a supplementary table that records the longitude and latitude, water depth, and sampling site color, along with other relevant information.

These bacterial strains were isolated from habitats such as ice, snow, and lake water on the Qinghai-Tibet Plateau. The colonies exhibit distinct colors, which likely correlate with their adaptation to the harsh environmental conditions of the plateau, including strong ultraviolet radiation, low temperatures, and oligotrophic environments, achieved through mechanisms like differential pigment production. The colonies were isolated via spread plating using lake water samples, incubated at 4℃ on media including R2A agar, and obtained after confirming no contamination and pure single colonies. Subsequent analysis will include full-length 16S rRNA gene sequencing of the purified single colonies, with a 97% sequence similarity threshold used to determine if the strain represents a novel species. If the sequence similarity between the obtained 16S rRNA gene sequence and that of its closest matched strain is lower than 97%, whole-genome sequencing will be performed to clarify the strain's phylogenetic relationship within its corresponding genus, family, or even phylum, confirm whether the strain has a novel phylogenetic status, and enrich the microbial diversity dataset of lakes on the Qinghai-Tibet Plateau.