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In vivo examination of membrane protein localization and degradation with green fluorescent protein.

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PubMed Central1996-01-23 更新2026-05-02 收录
下载链接:
https://pmc.ncbi.nlm.nih.gov/articles/PMC40142/
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资源简介:
To test the utility of green fluorescent protein (GFP) as an in vivo reporter protein when fused to a membrane domain, we made a fusion protein between yeast hydroxymethylglutaryl-CoA reductase and GFP. Fusion proteins displayed spatial localization and regulated degradation consistent with the native hydroxymethylglutaryl-CoA reductase proteins. Thus, GFP should be useful in the study of both membrane protein localization and protein degradation in vivo. IMAGES:
提供机构:
National Academy of Sciences
创建时间:
1996-01-23
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