Lead peptides for the molecular evolution process identified from a panel of alleged G_M1-binding peptides.

Fitness values correspond to fluorescence intensities obtained in a solid-phase binding assay with lysoGM1/DY650-conjugate (arbitrary units) and reflect the probe-binding strength of each peptide. Highlighted amino acids were identified in “alanine walk” experiments as beneficial (boldface) or detrimental (underlined) for binding.