Cardiac Lysosomal Organisation During Ageing: IVIS Imaging, qPCR Analysis, and R-Based Statistical Models

This dataset supports a research project investigating age-associated changes in cardiac lysosomal organisation using a combination of IVIS Lysotracker imaging, qPCR-based gene expression profiling, and R-based statistical modelling. The study aimed to determine whether ageing alters the abundance of acidic vesicles or the acidification machinery in murine hearts.Whole-heart acidic vesicle signals were quantified using IVIS two-dimensional (2D) fluorescence imaging from both anterior and posterior orientations, enabling rapid acquisition (~10 seconds per heart). RT-qPCR analyses were performed for lysosomal and autophagy-related markers, including Lamp2, Atp6v1a, Sqstm1, Cd63, and M6pr, to evaluate transcriptional remodelling. R scripts were used to perform linear mixed-effects modelling to assess the effects of age, region, and batch on imaging and qPCR results.The repository includes raw fluorescence intensity data, qPCR cycle threshold (Ct) values, and all scripts used for statistical analysis. These data support the reproducibility of the study and provide a resource for researchers interested in cardiac ageing, lysosomal biology, vesicular trafficking, and imaging-based quantification of acidic compartments.

本数据集支持一项研究项目，该项目结合IVIS Lysotracker成像、基于qPCR的基因表达谱分析以及基于R语言的统计建模，探究心脏溶酶体组织的年龄相关变化。本研究旨在明确衰老是否会改变小鼠心脏中酸性囊泡的丰度或酸化相关机制。通过从前后两个方位采集的IVIS二维（2D）荧光成像，对全心脏酸性囊泡信号进行定量分析，实现单颗心脏约10秒的快速成像采集。针对溶酶体及自噬相关标志物（包括Lamp2、Atp6v1a、Sqstm1、Cd63及M6pr）开展RT-qPCR分析，以评估转录重塑情况。使用R语言脚本开展线性混合效应建模，以评估年龄、区域及批次对成像及qPCR实验结果的影响。本数据集仓库包含原始荧光强度数据、qPCR循环阈值（Ct）值以及所有用于统计分析的脚本文件。本数据集可为研究的可重复性验证提供支撑，并可为关注心脏衰老、溶酶体生物学、囊泡运输以及基于成像的酸性区室定量分析的研究人员提供研究资源。