Imaging dataset 01 for mtFociCounter
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This is the first dataset associated with the updated manuscript on mtFociCounter. Images are Spinning Disk Confocal Images of unsorted 3t3 NIH mouse fibroblasts stably expressing <strong>mitochondrially</strong> targeted dsRed, immunofluorescence against dsDNA (mitochondrial <strong>nucleoids</strong>) and AlexaFluor 647, and stained <strong>nuclei</strong> with Hoechst. It contains all data necessary to reproduce the analysis of 3t3 WT sampling (sample-to-sample comparison), as described in the manuscript. "samples" from the same day are 13mm coverslips processed in parallel (seeding of cells, fixation, immunofluorescence) and mounted on the same glass slide (#1 on left, #2 centre, #3 right). The dataset contains the 3t3 WT data from the following acquisition dates: 20220603_sample2<br> 20220603_sample3<br> 20220706_sample2<br> 20220706_sample3<br> 20220708_sample1<br> 20220708_sample2<br> 20220708_sample3<br> 20221022_sample2<br> 20221022_sample3 For details of the sampling procedure, please refer to the accompanying manuscript, which will soon be made available on BioRxiv.<br>



